Couraud P O, Lu B Z, Schmutz A, Durieu-Trautmann O, Klutchko-Delavier C, Hoebeke J, Strosberg A D
J Cell Biochem. 1983;21(3):187-93. doi: 10.1002/jcb.240210301.
Two types for antibodies have been raised against the beta-adrenergic receptor: either by injection of highly purified receptor from turkey erythrocytes or by injection of anticatecholamine ligand antibodies, and induction of anti-idiotypic antibodies. Our data illustrate the interactions of the beta-adrenergic receptor with these polyclonal antibodies. Preliminary results with monoclonal antibodies are also described. The redistribution of beta-receptors on intact cells is visualized by the use of fluorescent antibodies. Immunoprecipitation of radioiodinated receptor by the antireceptor antibodies yields a single major 60,000 MW component.
已经产生了两种针对β-肾上腺素能受体的抗体：一种是通过注射从火鸡红细胞中高度纯化的受体，另一种是通过注射抗儿茶酚胺配体抗体并诱导抗独特型抗体。我们的数据阐明了β-肾上腺素能受体与这些多克隆抗体的相互作用。还描述了单克隆抗体的初步结果。通过使用荧光抗体可观察到完整细胞上β受体的重新分布。抗受体抗体对放射性碘化受体的免疫沉淀产生一个单一的主要60000分子量成分。
