Bovine viral diarrhea virus: purification of surface proteins in detergent-containing buffers by fast protein liquid chromatography.

Bovine viral diarrhea virus was purified by lectin chromatography. The glycoprotein peplomers were dissociated from the virion by treatment with detergent. By a second lectin gel chromatography the glycoconjugates containing terminal galactose were prepared. In combination with lectin affinity chromatography, ion-exchange chromatography on Mono-Q in the presence of the low-UV-absorbing detergent Berol 172 proved to be a powerful technique both for analytical and preparative applications.