Mendelovitz S, Aharonowitz Y
J Gen Microbiol. 1983 Jul;129(7):2063-9. doi: 10.1099/00221287-129-7-2063.
This work describes isolation and characterization of Streptomyces clavuligerus mutants resistant to the lysine analogue S-(2-aminoethyl)-L-cysteine (AEC). The mutation to AEC resistance was shown to affect the feedback regulation of aspartokinase; 70% of the mutants isolated had aspartokinase activity insensitive to concerted feedback inhibition by lysine plus threonine. Among these mutants, 70% (about 50% of the total AEC-resistant strains isolated) showed significant overproduction of beta-lactam antibiotics.
这项工作描述了克拉维链霉菌对赖氨酸类似物S-(2-氨基乙基)-L-半胱氨酸（AEC）具有抗性的突变体的分离和特性鉴定。对AEC抗性的突变被证明会影响天冬氨酸激酶的反馈调节；分离得到的突变体中有70%的天冬氨酸激酶活性对赖氨酸加苏氨酸的协同反馈抑制不敏感。在这些突变体中，70%（约占分离得到的AEC抗性菌株总数的50%）显示出β-内酰胺抗生素的显著过量生产。
