克拉维链霉菌的调节突变体在游离二氨基庚二酸含量和抗生素生物合成方面受到影响。
Regulatory mutants of Streptomyces clavuligerus affected in free diaminopimelic acid content and antibiotic biosynthesis.
作者信息
Aharonowitz Y, Mendelovitz S, Kirenberg F, Kuper V
出版信息
J Bacteriol. 1984 Jan;157(1):337-40. doi: 10.1128/jb.157.1.337-340.1984.
Abstract
The composition of intracellular free amino acid pools was determined in Streptomyces clavuligerus mutants possessing an altered aspartokinase which is insensitive to concerted feedback inhibition by threonine and lysine. These mutants contained total free amino acid pool contents that were considerably higher than those found in the wild-type strain. Diaminopimelic acid accounted for 10 to 20% of the total free amino acid pools, depending on the individual mutant and its culture growth phase, whereas diaminopimelic acid contained in the wild-type strain accounted for only 0.5% of the total free amino acid pool.
摘要
在拥有一种改变的天冬氨酸激酶的棒状链霉菌突变体中测定了细胞内游离氨基酸库的组成，该天冬氨酸激酶对苏氨酸和赖氨酸的协同反馈抑制不敏感。这些突变体的总游离氨基酸库含量明显高于野生型菌株。二氨基庚二酸占总游离氨基酸库的10%至20%，这取决于单个突变体及其培养生长阶段，而野生型菌株中含有的二氨基庚二酸仅占总游离氨基酸库的0.5%。
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本文引用的文献
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beta-lactam antibiotic production by Streptomyces clavuligerus mutants impaired in regulation of aspartokinase.克拉维链霉菌中天冬氨酸激酶调控受损的突变体产生β-内酰胺抗生素的情况
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