Romert L, Jenssen D
Mutat Res. 1983 Oct;111(2):245-52. doi: 10.1016/0027-5107(83)90067-2.
Rabbit pulmonary alveolar macrophages (PAM) were used as a metabolizing device in combination with V79 Chinese hamster cells as a mutational indicator system. The capacity for metabolic activation of benzo[a]pyrene (B[a]P), its 7,8-diol and 2-aminoanthracene by PAM was investigated. Because of the high variation between different PAM preparations, a statistically significant effect of the 3 compounds could only be demonstrated in a series of 4 or 5 experiments. When the ability of PAM to metabolize B[a]P and the 7,8-diol to mutagenic products was compared with that of primary embryonic fibroblasts from Syrian hamsters, PAM were found to be one-tenth as efficient. Experiments were performed to find out how the phagocytic process could affect the metabolic activation of polycyclic aromatic hydrocarbons. The results showed that PAM-mediated mutagenesis of the 7,8-diol was enhanced 5-10-fold if PAM were fed with opsonized particles. The mechanism by which the phagocytosis of PAM enhanced the mutagenicity of 7,8-diol, as detected in co-cultivated V79 cells, can so far only be a matter for speculation.
兔肺泡巨噬细胞（PAM）被用作代谢装置，与V79中国仓鼠细胞一起作为突变指示系统。研究了PAM对苯并[a]芘（B[a]P）、其7,8-二醇和2-氨基蒽的代谢活化能力。由于不同PAM制剂之间差异很大，只有在一系列4或5次实验中才能证明这3种化合物具有统计学意义的效果。当将PAM代谢B[a]P和7,8-二醇生成诱变产物的能力与叙利亚仓鼠原代胚胎成纤维细胞的能力进行比较时，发现PAM的效率只有前者的十分之一。进行实验以探究吞噬过程如何影响多环芳烃的代谢活化。结果表明，如果给PAM喂食调理过的颗粒，PAM介导的7,8-二醇诱变作用会增强5至10倍。就共培养的V79细胞中检测到的情况而言，PAM的吞噬作用增强7,8-二醇诱变性的机制目前只能靠推测。
