Weinert T A, Schaus N A, Grindley N D
Science. 1983 Nov 18;222(4625):755-65. doi: 10.1126/science.6314502.
Insertion sequences (IS) are discrete segments of DNA that can transpose from one genomic site to another and promote genetic rearrangements. A question that is central to understanding the mechanism of transpositional recombination is whether genetic rearrangements are accompanied by duplication of the IS that promotes them. Analysis of adjacent deletions mediated by IS903 provides the strongest evidence to date than any IS-mediated transpositional recombination can occur by an efficient replicative mechanism.
插入序列（IS）是DNA的离散片段，可从一个基因组位点转座到另一个位点并促进基因重排。理解转座重组机制的核心问题是基因重排是否伴随着促进其发生的IS的重复。对由IS903介导的相邻缺失的分析提供了迄今为止最有力的证据，表明任何IS介导的转座重组都可以通过高效的复制机制发生。
