从百日咳博德特氏菌中分离出的重复序列会高频诱导DNA重排和缺失。
Repeated sequences isolated from Bordetella pertussis induce DNA rearrangements and deletions at high frequency.
作者信息
Nechaeva E V, Butcher S, Sinjashina L, Runeberg K, Romantschuk M, Karataev G I
机构信息
Gamaleya Institute for Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.
出版信息
Gene. 1995 Dec 1;166(1):111-6. doi: 10.1016/0378-1119(95)00554-3.
Two repeated sequences (RS) from Bordetella pertussis were cloned in Escherichia coli and sequenced. The RS, called RSBP1 and RSBP3, are highly homologous to other B. pertussis RS. The recombinant plasmids containing RSBP1 and RSBP3 or transposon-like structures of these elements were not stable but segregated plasmids with deletions or rearranged DNA. RS of B. pertussis seem to be able to stimulate both intra- and inter-genomic RecA-independent recombination events. In at least one case, the observed deletion had occurred precisely between the RS terminus and a site with sequence homology to the terminus. The high frequency rearrangements associated with the RS imply that the RS are transposable elements.
从百日咳博德特氏菌中克隆出两个重复序列（RS），并在大肠杆菌中进行测序。这两个RS分别称为RSBP1和RSBP3，与其他百日咳博德特氏菌的RS高度同源。含有RSBP1和RSBP3或这些元件的转座子样结构的重组质粒不稳定，会分离出有缺失或DNA重排的质粒。百日咳博德特氏菌的RS似乎能够刺激基因组内和基因组间不依赖RecA的重组事件。在至少一个案例中，观察到的缺失恰好发生在RS末端和与末端具有序列同源性的位点之间。与RS相关的高频重排表明RS是可移动元件。
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