一名日本患者伯基特淋巴瘤的爱泼斯坦-巴尔病毒阴性B淋巴瘤细胞系（THP-2）

An Epstein-Barr virus-negative B lymphoma cell line (THP-2) from a Burkitt's lymphoma of a Japanese patient.

作者信息

Tsuchiya S, Yamaguchi Y, Kobayashi Y, Minegishi M, Imaizumi M, Suzuki H, Konno T, Tada K

出版信息

Tohoku J Exp Med. 1983 Aug;140(4):429-34. doi: 10.1620/tjem.140.429.

DOI:10.1620/tjem.140.429

Abstract

An Epstein-Barr virus-negative lymphoma cell line, THP-2, was established from a Burkitt's lymphoma taken from a Japanese patient. THP-2 cells grew in single cell suspension with a doubling time of 24 hr; the cells carried surface-bound mu-lambda immunoglobulins and formed rosettes with IgG antibody-coated ox erythrocytes. THP-2 cells expressed B1, common acute lymphoblastic leukemia (ALL) (J5) and Ia-like antigens of their surface as defined by monoclonal antibodies. Epstein-Barr virus-associated nuclear antigen (EBNA) was not detected. The cell line THP-2 has been maintained for over 5 years. Among the markers examined, only common ALL antigen has been present on the cell surface of THP-2 for these 5 years.

摘要

一种爱泼斯坦-巴尔病毒阴性淋巴瘤细胞系THP-2，是从一名日本患者的伯基特淋巴瘤中建立的。THP-2细胞以单细胞悬液形式生长，倍增时间为24小时；这些细胞携带表面结合的μ-λ免疫球蛋白，并与IgG抗体包被的牛红细胞形成玫瑰花结。THP-2细胞表面表达由单克隆抗体定义的B1、普通急性淋巴细胞白血病（ALL）（J5）和Ia样抗原。未检测到爱泼斯坦-巴尔病毒相关核抗原（EBNA）。细胞系THP-2已维持了5年多。在检测的标志物中，在这5年里只有普通ALL抗原一直存在于THP-2细胞表面。