Solianik R G, Karpova E F, Tsilinskiĭ Ia Ia, Tymchishin P N
Vopr Virusol. 1983 Jul-Aug;28(4):50-3.
The main molecular biology parameters of an attenuated mutant DMS-20/6 of eastern equine encephalomyelitis virus derived by treatment with dimethylsulphate of the wild type virus (strain No. 627) were determined. The sedimentation coefficient of sucrose density gradient purified and concentrated virus was 280 S, the buoyant density of virions in sucrose density gradient was 1.19 g/cm3. The DMS-20/6 virion had 3 proteins with molecular weights of 56, 50, and 34 kilodaltons, and the size of virions by negative staining was 58-77 nm.
测定了用硫酸二甲酯处理野生型病毒（627株）得到的东部马脑脊髓炎病毒减毒突变体DMS-20/6的主要分子生物学参数。经蔗糖密度梯度纯化和浓缩后的病毒沉降系数为280S，病毒粒子在蔗糖密度梯度中的浮力密度为1.19g/cm³。DMS-20/6病毒粒子有3种分子量分别为56、50和34千道尔顿的蛋白质，负染法测得病毒粒子大小为58 - 77nm。
