Dubinina G P, Basnak'ian I A, Tsvetkova N V, Semina I E, Tarasov A V
Zh Mikrobiol Epidemiol Immunobiol. 1983 Sep(9):12-7.
The present article deals with the study of the multicyclic process of the cultivation of B. amyloliquefaciens in a liquid medium prepared from natural raw materials and with obtaining restrictase Bam H1 from the biomass with different microbiological characteristics. The selected medium has been found to be suitable for the realization of the multicyclic cultivation process on an industrial scale. The tendency towards a drop in the level of nonspecific nucleases simultaneously with a decrease in the specific growth rate has been revealed. A simplified scheme for the purification of the restricting endonuclease is proposed.
本文涉及对解淀粉芽孢杆菌在由天然原料制备的液体培养基中进行多循环培养过程的研究，以及从具有不同微生物学特性的生物质中获取限制性内切酶Bam H1的研究。已发现所选培养基适用于在工业规模上实现多循环培养过程。揭示了非特异性核酸酶水平下降与比生长速率降低同时出现的趋势。提出了一种简化的限制性内切核酸酶纯化方案。
