Perel'man E V, Shtanchaeva S M, Bulk V F, Tarasov A P, Bakh N L
Zh Mikrobiol Epidemiol Immunobiol. 1984 Dec(12):48-50.
The possibility of using culture media prepared from local ingredients and intended for growing the producers of restricting enzyme Xba 1 has been demonstrated. The yield of restricting enzyme Xba 1 per g of crude biomass, obtained with the use of peptone-yeast medium prepared from ingredients supplied by Difco Laboratories (USA), has proved to be 4 times greater than that obtained with the use of peptone-yeast medium prepared from local ingredients. At the same time the use of casein-saline medium ensures the yield of the enzyme, similar to that obtained with the use of peptone-yeast medium prepared from ingredients supplied by Difco Laboratories, but with a greater content of nonspecific nucleases.
已经证明了使用由当地成分制备的、用于培养限制性内切酶Xba 1产生菌的培养基的可能性。使用由美国Difco实验室提供的成分制备的蛋白胨 - 酵母培养基，每克粗生物质获得的限制性内切酶Xba 1产量，已证明比使用由当地成分制备的蛋白胨 - 酵母培养基获得的产量高4倍。同时，使用酪蛋白 - 盐溶液培养基可确保酶的产量，与使用由Difco实验室提供的成分制备的蛋白胨 - 酵母培养基所获得的产量相似，但非特异性核酸酶的含量更高。
