Fagin K D, Shinsako J, Dallman M F
Am J Physiol. 1983 Nov;245(5 Pt 1):E515-20. doi: 10.1152/ajpendo.1983.245.5.E515.
Effects of housing and cannulation on plasma ACTH and corticosterone levels were evaluated to validate a method for obtaining repeated blood samples for ACTH from conscious rats. Resting morning, evening, or noise-stimulated (4 min of metal rattling) levels of plasma ACTH or corticosterone obtained by decapitation were similar in rats housed alone or in pairs. Individually housed, cannulated rats had slightly elevated morning levels of plasma ACTH and corticosterone, but the evening and noise-stimulated increases in hormone levels were similar to those in unoperated, decapitated rats. A hemorrhage stimulus administered on separate days to cannulated rats evoked highly replicable elevations in plasma ACTH and corticosterone. These results demonstrate that 1) ACTH and corticosterone secretion are similar in rats housed alone versus in pairs, and 2) long-term cannulation permits multiple intra-animal comparisons of basal and acutely stimulated plasma ACTH and corticosterone levels that are useful reflections of those measured in decapitated rats.
评估了饲养方式和插管对血浆促肾上腺皮质激素（ACTH）和皮质酮水平的影响，以验证一种从清醒大鼠获取重复ACTH血样的方法。单独饲养或成对饲养的大鼠，断头法获取的静息状态下早晨、傍晚或噪音刺激（4分钟金属碰撞声）时的血浆ACTH或皮质酮水平相似。单独饲养的插管大鼠早晨血浆ACTH和皮质酮水平略有升高，但傍晚和噪音刺激引起的激素水平升高与未手术断头大鼠相似。在不同日期对插管大鼠施加出血刺激可引起血浆ACTH和皮质酮高度可重复的升高。这些结果表明：1）单独饲养与成对饲养的大鼠ACTH和皮质酮分泌相似；2）长期插管允许在动物体内多次比较基础状态和急性刺激状态下的血浆ACTH和皮质酮水平，这些比较能有效反映断头大鼠所测水平。
