Weber A F, Schmerr M J, Sorensen D K, Bingham C, Pomeroy K, Miller J M, Van Der Maaten M J
Am J Vet Res. 1983 Oct;44(10):1912-5.
The infectivity for the bovine leukemia virus (BLV) of bovine blood lymphocytes, with varying lymphocyte nuclear pocket (LNP) prevalence, was studied by intradermal injection of buffy coat leukocytes from cows into sheep. Thirty-five sheep were assigned to 3 groups of 9, 11, and 15, respectively, as recipients of blood leukocytes from individual cows as follows: 9 leukocyte donor cows included 3 BLV-seropositive cows with high (greater than or equal to 2.0%) LNP prevalence (group 1); 3 BLV-seropositive cows with low (less than 0.5%) LNP prevalence (group 2); and 3 BLV-seronegative cows with increased (greater than or equal to 0.5%) LNP prevalence (group 3). The 9 sheep in group 1 became BLV-seropositive by postinoculation day (PID) 57 as determined by the agar-gel immunodiffusion test; 2 of 11 sheep in group 2 became agar-gel immunodiffusion seropositive by PID 40; none of the 15 sheep of group 3 became seropositive by PID 92. Seemingly, there is a real difference in infectivity among BLV-seropositive cattle which is related to LNP prevalence, and seronegative cattle with increased LNP prevalence may be noninfectious, ie, were not carrying covert BLV virogene.
通过将奶牛的血沉棕黄层白细胞皮内注射到绵羊体内，研究了淋巴细胞核袋（LNP）患病率不同的牛血液淋巴细胞对牛白血病病毒（BLV）的感染性。35只绵羊被分为3组，分别为9只、11只和15只，作为个体奶牛血液白细胞的接受者，具体如下：9只白细胞供体奶牛包括3只LNP患病率高（大于或等于2.0%）的BLV血清阳性奶牛（第1组）；3只LNP患病率低（小于0.5%）的BLV血清阳性奶牛（第2组）；以及3只LNP患病率升高（大于或等于0.5%）的BLV血清阴性奶牛（第3组）。通过琼脂凝胶免疫扩散试验测定，第1组的9只绵羊在接种后天数（PID）57时变为BLV血清阳性；第2组11只绵羊中有2只在PID 40时变为琼脂凝胶免疫扩散血清阳性；第3组的15只绵羊在PID 92时均未变为血清阳性。显然，BLV血清阳性牛之间的感染性存在实际差异，这与LNP患病率有关，LNP患病率升高的血清阴性牛可能无感染性，即未携带隐性BLV病毒基因。
