Pal B K, Mohan S, Nimo R, Gardner M B
Arch Virol. 1983;77(2-4):239-47. doi: 10.1007/BF01309271.
Ecotropic wild mouse retrovirus (1504 M)-induced neurogenic paralytic disease has been studied in inbred strains of mice. The major criterion for the successful transmission of the disease in the laboratory strains of mice is inoculation of high titer ecotropic virus in FV-1n strains of mice at newborn stage (less than or equal to 1 day old), Hybridization studies using 1504 M viral cDNA as probe indicate that in nonparalyzed mice, the inoculated virus replicates primarily in spleen tissue, whereas virus replication is evident in both spleen and central nervous system (CNS) tissue of paralyzed mice. Our studies on virus gene expression indicate that both viral gag gene product p30 and env gene product gp70 are expressed in brain, spinal cord and spleen tissues of paralyzed mice. Together, these results indicate that inoculation of neurotropic wild mouse virus into FV-1n strains of newborn laboratory mice is necessary for the establishment of infection in CNS tissue leading to virus replication and expression and resulting in the paralytic disease.
已在近交系小鼠中研究了嗜亲性野生小鼠逆转录病毒（1504 M）诱导的神经源性麻痹性疾病。在实验室小鼠品系中成功传播该疾病的主要标准是在新生阶段（小于或等于1日龄）向FV-1n品系小鼠接种高滴度嗜亲性病毒。使用1504 M病毒cDNA作为探针的杂交研究表明，在未瘫痪的小鼠中，接种的病毒主要在脾脏组织中复制，而在瘫痪小鼠的脾脏和中枢神经系统（CNS）组织中病毒复制均很明显。我们对病毒基因表达的研究表明，病毒gag基因产物p30和env基因产物gp70在瘫痪小鼠的脑、脊髓和脾脏组织中均有表达。总之，这些结果表明，将嗜神经性野生小鼠病毒接种到新生实验室小鼠的FV-1n品系中对于在CNS组织中建立感染、导致病毒复制和表达并引发麻痹性疾病是必要的。
