用于检测抗牛白血病病毒抗体的补体诱导合胞体抑制试验
Syncytium-induction inhibition test with complement for detection of antibodies against bovine leukemia virus.
作者信息
Kono Y, Irishio W, Sentsui H
出版信息
Can J Comp Med. 1983 Jul;47(3):328-31.
A modified syncytium-induction inhibition test which is more sensitive than the immunodiffusion test, was developed using rabbit complement. In this test, fetal lamb kidney cells continuously infected with bovine leukemia virus were used as effector cells, and the CC81 cat cells transformed with murine sarcoma virus, were used as indicator cells. The syncytium-induction inhibition effect of anti-bovine leukemia virus serum was enhanced significantly by the addition of rabbit complement. The syncytium-induction inhibition titers had a statistically significant correlation with the immunodiffusion titers and were four to 64 times higher than immunodiffusion titers. In 12 experimentally infected cattle, the syncytium-induction inhibition test detected the antibodies earlier than the immunodiffusion test and continuously detected them when immunodiffusion antibody changed to negative. In the 81 sera from naturally infected herds, 35 (43.2%) were positive by the immunodiffusion test and 55 (67.9%) by the syncytium-induction inhibition test.
利用兔补体开发了一种改良的合胞体诱导抑制试验，该试验比免疫扩散试验更灵敏。在该试验中，将持续感染牛白血病病毒的胎羊肾细胞用作效应细胞，将用鼠肉瘤病毒转化的CC81猫细胞用作指示细胞。加入兔补体后，抗牛白血病病毒血清的合胞体诱导抑制作用显著增强。合胞体诱导抑制效价与免疫扩散效价具有统计学显著相关性，且比免疫扩散效价高4至64倍。在12头实验感染牛中，合胞体诱导抑制试验比免疫扩散试验更早检测到抗体，并且在免疫扩散抗体变为阴性时仍能持续检测到。在来自自然感染牛群的81份血清中，免疫扩散试验有35份（43.2%）呈阳性；合胞体诱导抑制试验有55份（67.9%）呈阳性。
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