Ultrastructural and ultrahistochemical investigations of lead-induced intranuclear inclusion bodies in rat kidney.

The morphogenesis of intranuclear inclusion bodies in the proximal convoluted tubules of rat kidney is studied in chronic lead poisoning. In early stages electron dense particles and fibrillar structures in connection with interchromatin granules were observed. The fully developed inclusion bodies showed an electron dense core from which fibrils project outwards forming an irregular coronal framework. By the sulphide silver method for the demonstration of heavy metals an intense reaction was observed in the outer areas of the fibrillar corona after digestion using DNase.