Ikenberg H, Gissmann L, Gross G, Grussendorf-Conen E I, zur Hausen H
Int J Cancer. 1983 Nov 15;32(5):563-5. doi: 10.1002/ijc.2910320507.
32P-labelled DNA of HPV 16 which has been isolated and molecularly cloned from a cervical carcinoma (Dürst et al., 1983) was used to screen the cellular DNAs obtained from 20 different biopsies of Morbus Bowen or Bowenoid papulosis, respectively, by Southern blot analysis. Under conditions of differing stringency for the hybridization, HPV 16 DNA or related sequences were identified in 6 out of 10 cases of Morbus Bowen (4 out of 5 from a genital localization) and in 8 out of 10 biopsies from Bowenoid papulosis. One additional case of the latter disease contained DNA sequences of an HPV type not yet classified. There is evidence for the presence of another HPV DNA in two of the HPV-16-positive tumors. A large number of normal genital tissue samples were negative for HPV DNA.
从宫颈癌中分离并进行分子克隆的人乳头瘤病毒16型（HPV 16）的32P标记DNA（Dürst等人，1983年），用于通过Southern印迹分析筛选分别从20例不同的鲍温病或鲍温样丘疹病活检组织中获得的细胞DNA。在不同严格程度的杂交条件下，在10例鲍温病病例中的6例（5例来自生殖器部位的病例中有4例）以及10例鲍温样丘疹病活检组织中的8例中鉴定出HPV 16 DNA或相关序列。后一种疾病的另外1例含有尚未分类的HPV类型的DNA序列。有证据表明，在2例HPV - 16阳性肿瘤中存在另一种HPV DNA。大量正常生殖器组织样本的HPV DNA检测为阴性。
