Nagle W A, Moss A J
Int J Radiat Biol Relat Stud Phys Chem Med. 1983 Nov;44(5):475-81. doi: 10.1080/09553008314551471.
Two inhibitors of poly(ADP-ribose) synthetase, 5-methylnicotinamide and m-methoxybenzamide, enhanced the cytotoxicity of 42 degrees C and 45 degrees C hyperthermia in cultured Chinese hamster V79 cells. The inhibitors showed minimal toxicity for cells treated at 37 degrees C, and did not appreciably alter cellular ATP levels under any of the experimental conditions used. Enhanced cell killing occurred when the inhibitors were added after an acute (5-10 min) 45 degrees C heat shock, and after 50 and 100 min exposures to 42 degrees C. When present during heating at 42 degrees C, the inhibitors reduced the shoulder of the 42 degrees C survival curves but did not appreciably affect the slopes. The results suggest a possible role for poly(ADP-ribose) synthetase in the survival response of V79 cells to hyperthermia.
两种聚（ADP - 核糖）合成酶抑制剂，5 - 甲基烟酰胺和间甲氧基苯甲酰胺，增强了42℃和45℃热疗对培养的中国仓鼠V79细胞的细胞毒性。这些抑制剂对在37℃处理的细胞显示出最小的毒性，并且在任何所用实验条件下都没有明显改变细胞ATP水平。当在急性（5 - 10分钟）45℃热休克后以及在暴露于42℃50和100分钟后加入抑制剂时，细胞杀伤增强。当在42℃加热期间存在时，抑制剂减少了42℃存活曲线的肩部，但没有明显影响斜率。结果表明聚（ADP - 核糖）合成酶在V79细胞对热疗的存活反应中可能起作用。
