Asaka M, Nagase K, Alpert E
Isozymes Curr Top Biol Med Res. 1983;11:183-95.
Radioimmunoassays specific for ALD isozymes were developed for the quantification of human ALD-A, -B, and -C. The method is a double antibody radioimmunoassay consisting of purified radioiodinated ALD-A, -B, and C as ligand, chicken antibodies to ALD-A, -B, and -C, and rabbit antibodies to chicken IgG. The Iodogen method was used for the iodination of the purified isozymes. ALD-A was present in high concentration in muscle, ALD-B in adult liver, and ALD-C in adult brain. ALD-A was elevated in hepatoma tissue and hepatoma cell lines, whereas ALD-B was distinctly low. Normal serum levels for the three isozymes were determined. The ALD-A level in the serum from 41 normal subjects was 170 +/- 39 ng/ml. Serum ALD-A level was increased in many patients with cancer and muscle diseases, but not in patients with hepatitis or other benign diseases. Serum ALD-B level in 11 normal subjects was 28.5 +/- 9.2 ng/ml. Serum ALD-C level in 12 normal subjects was 2.4 +/- 0.7 ng/ml. The determination of ALD-A, -B, and -C by radioimmunoassay may be a valuable tool in biochemical and clinical studies of these isozymes.
已开发出针对醛缩酶（ALD）同工酶的放射免疫测定法，用于定量检测人ALD-A、-B和-C。该方法是一种双抗体放射免疫测定法，由纯化的放射性碘化ALD-A、-B和-C作为配体、针对ALD-A、-B和-C的鸡抗体以及针对鸡免疫球蛋白G的兔抗体组成。采用碘代甘氨酸法对纯化的同工酶进行碘化。ALD-A在肌肉中浓度较高，ALD-B在成人肝脏中，ALD-C在成人脑中。ALD-A在肝癌组织和肝癌细胞系中升高，而ALD-B明显较低。测定了三种同工酶的正常血清水平。41名正常受试者血清中ALD-A水平为170±39 ng/ml。许多癌症和肌肉疾病患者血清ALD-A水平升高，但肝炎或其他良性疾病患者未升高。11名正常受试者血清ALD-B水平为28.5±9.2 ng/ml。12名正常受试者血清ALD-C水平为2.4±0.7 ng/ml。通过放射免疫测定法测定ALD-A、-B和-C可能是这些同工酶生化和临床研究中的一种有价值的工具。
