Asaka M, Alpert E
Ann N Y Acad Sci. 1983;417:359-67. doi: 10.1111/j.1749-6632.1983.tb32878.x.
Radioimmunoassays specific for fructose-1, 6-diphosphate aldolase isozymes were developed for the quantification of human aldolase A, B and C. The method is a double-antibody radioimmunoassay using radioiodinated purified aldolase A, B and C as ligand, chicken antibodies to aldolase A, B and C, and rabbit antibodies to chicken IgG. The Iodogen method was used for the iodination of aldolase A, B and C in this study. Aldolase A was predominantly high in concentration in muscle, aldolase B was high in normal adult liver, and aldolase C was high in adult brain. Aldolase A was elevated in hepatoma tissue and hepatoma cell lines, where aldolase B was distinctly low. Normal serum levels for the three isozymes were determined. The aldolase A levels in serum obtained from 41 normal subjects were 170 +/- 39 ng/ml. Serum aldolase A levels were increased in many patients with cancer and muscle diseases, but were not increased in patients with hepatitis or other benign diseases. Serum aldolase B levels obtained from 11 normal subjects were 28.5 +/- 9.2 ng/ml. Serum aldolase B levels were increased in patients with hepatitis and correlated well with serum GPT levels. Serum aldolase C levels obtained from 12 normal subjects were 2.4 +/- 0.7 ng/ml. The determination of aldolase A, B and C by radioimmunoassay may be a valuable tool in biochemical and clinical studies of aldolase isozymes.
开发了针对果糖-1,6-二磷酸醛缩酶同工酶的放射免疫测定法，用于定量检测人醛缩酶A、B和C。该方法是一种双抗体放射免疫测定法，使用放射性碘化的纯化醛缩酶A、B和C作为配体，针对醛缩酶A、B和C的鸡抗体，以及针对鸡IgG的兔抗体。本研究采用碘代甘氨酸法对醛缩酶A、B和C进行碘化。醛缩酶A在肌肉中的浓度主要较高，醛缩酶B在正常成人肝脏中含量较高，醛缩酶C在成人大脑中含量较高。醛缩酶A在肝癌组织和肝癌细胞系中升高，而醛缩酶B明显较低。测定了三种同工酶的正常血清水平。41名正常受试者血清中的醛缩酶A水平为170±39 ng/ml。许多癌症和肌肉疾病患者的血清醛缩酶A水平升高，但肝炎或其他良性疾病患者的血清醛缩酶A水平未升高。11名正常受试者血清中的醛缩酶B水平为28.5±9.2 ng/ml。肝炎患者的血清醛缩酶B水平升高，且与血清谷丙转氨酶水平密切相关。12名正常受试者血清中的醛缩酶C水平为2.4±0.7 ng/ml。通过放射免疫测定法测定醛缩酶A、B和C可能是醛缩酶同工酶生化和临床研究中的一种有价值的工具。
