Samelson L E, Schwartz R H
J Immunol. 1983 Dec;131(6):2645-50.
Pigeon cytochrome c-specific, IL 2-secreting T cell hybrids have been used for immunizations to generate alloantibodies against the T cell antigen receptor on these cells. The B10.A-derived cloned T cell hybrid 2B4 was emulsified in complete Freund's adjuvant and injected i.p. into several F1 strains of mice. After boosting the recipient animals with cells in incomplete Freund's adjuvant, malignant ascites developed. In the (BALB/c X AKR)F1 or (AKR X BALB/c)F1 strains, these ascites consistently contained material that specifically inhibited the antigen-induced IL 2 secretion of only the immunizing 2B4 cell. The inhibitory material was antibody that specifically bound and could be absorbed only by 2B4. A similar immunization was performed with the cell 2C2. Although this cell apparently has similar antigenic fine specificity as 2B4, high concentrations of ascites generated by 2C2 immune animals inhibited antigen-induced IL 2 release only from 2C2. At lower concentrations of ascites, this preparation synergized with antigen to increase the IL 2 release, again only from 2C2. This antibody preparation did not affect concanavalin A-induced IL 2 release. The most likely explanation for these data is that the ascites contain antibodies that react with the antigen-specific receptor on the T cell hybrids.
已使用鸽细胞色素c特异性、分泌白细胞介素2的T细胞杂交瘤进行免疫，以产生针对这些细胞上T细胞抗原受体的同种抗体。将源自B10.A的克隆T细胞杂交瘤2B4在完全弗氏佐剂中乳化，并腹腔注射到几种F1代小鼠品系中。在用不完全弗氏佐剂中的细胞对受体动物进行加强免疫后，出现了恶性腹水。在（BALB/c×AKR）F1或（AKR×BALB/c）F1品系中，这些腹水始终含有仅特异性抑制免疫的2B4细胞抗原诱导的白细胞介素2分泌的物质。抑制性物质是特异性结合且仅能被2B4吸收的抗体。用细胞2C2进行了类似的免疫。尽管该细胞显然具有与2B4相似的抗原精细特异性，但2C2免疫动物产生的高浓度腹水仅抑制2C2的抗原诱导的白细胞介素2释放。在腹水浓度较低时，该制剂与抗原协同作用以增加白细胞介素2释放，同样仅从2C2释放。该抗体制剂不影响伴刀豆球蛋白A诱导的白细胞介素2释放。对这些数据最可能的解释是，腹水中含有与T细胞杂交瘤上的抗原特异性受体反应的抗体。
