Ashwell J D, Cunningham R E, Noguchi P D, Hernandez D
J Exp Med. 1987 Jan 1;165(1):173-94. doi: 10.1084/jem.165.1.173.
Stimulation of antigen-specific T cell hybridomas with the appropriate antigen/MHC combination, at concentrations that resulted in the secretion of the lymphokine interleukin 2, resulted in a dose-dependent decrease in both [3H]thymidine incorporation and cell growth. Flow cytometric studies demonstrated that stimulation with antigen resulted in a cell cycle block that was most evident at the G1/S border, and mixing studies revealed that bystander T cells of different antigen specificities were not affected. For at least the large majority of T cells, the G1/S cell cycle block appeared to be irreversible after 24 h of exposure to antigen. This cell cycle block may be useful as a rapid and quantitative measure of T cell hybridoma activation, as a means of selecting T cell hybridomas that have functional alterations in the reception of stimulatory signals, and may serve as a model of the induction of tolerance in immature T cells.
用合适的抗原/MHC组合刺激抗原特异性T细胞杂交瘤，当浓度达到能分泌淋巴因子白细胞介素2时，会导致[3H]胸苷掺入量和细胞生长呈剂量依赖性下降。流式细胞术研究表明，抗原刺激导致细胞周期阻滞，在G1/S边界最为明显，混合研究显示不同抗原特异性的旁观者T细胞未受影响。对于至少绝大多数T细胞而言，暴露于抗原24小时后，G1/S细胞周期阻滞似乎是不可逆的。这种细胞周期阻滞可用作快速定量检测T细胞杂交瘤激活的方法，作为筛选在接受刺激信号方面有功能改变的T细胞杂交瘤的手段，还可能作为未成熟T细胞诱导耐受的模型。
