HPV-1、HPV-4和HPV-6诱发的病毒棘皮瘤中病毒DNA含量与成熟病毒颗粒证据之间的相关性

Correlation between content of viral DNA and evidence of mature virus particles in HPV-1, HPV-4, and HPV-6 induced virus acanthomata.

作者信息

Grussendorf-Conen E I, Gissmann L, Hölters J

出版信息

J Invest Dermatol. 1983 Dec;81(6):511-3. doi: 10.1111/1523-1747.ep12522845.

Abstract

In HPV-1 and HPV-4 induced warts as well as in HPV-6 positive condylomata acuminata the quantity of viral DNA encapsulated into virus particles was determined and compared to the total amount of viral DNA present in the papillomas. As shown by filter hybridization using 3H-labeled viral DNA molecularly cloned in Escherichia coli, the amount of total viral DNA found in HPV-1 or HPV-4 induced skin warts is similar. HPV-4 DNA, however, is encapsulated into virus particles with less efficiency. HPV-6 DNA can be detected only at minute amounts in condylomata acuminata and the percentage of DNA recovered from virions is extremely low.

摘要

在人乳头瘤病毒1型（HPV-1）和人乳头瘤病毒4型（HPV-4）诱发的疣以及HPV-6阳性尖锐湿疣中，测定了封装在病毒颗粒中的病毒DNA数量，并与乳头状瘤中存在的病毒DNA总量进行了比较。如使用在大肠杆菌中分子克隆的3H标记病毒DNA进行的滤膜杂交所示，在HPV-1或HPV-4诱发的皮肤疣中发现的总病毒DNA量相似。然而，HPV-4 DNA封装到病毒颗粒中的效率较低。在尖锐湿疣中只能检测到微量的HPV-6 DNA，从病毒粒子中回收的DNA百分比极低。