Viac J, Chardonnet Y, Bouvard V, Leval J, Morgon A, Thivolet J
Virchows Arch A Pathol Anat Histopathol. 1987;411(1):73-7. doi: 10.1007/BF00734517.
Thirty five non regressing cutaneous and mucosal human papillomas were studied for the expression of EGF and transferrin receptors by indirect immunofluorescence on frozen sections. The lesions were also examined for the presence of human papillomavirus (HPV) DNA by in situ-hybridization with biotinylated probes and viral capsid antigen. The mapping of EGF and transferrin receptors was modified in cutaneous lesions with drastic viral cytopathic effects and was enhanced in mucosal lesions mainly in laryngeal papillomas, which are poor virus producers. The greatest increase in EGF and transferrin receptor reactivity was observed in the group of mucosal lesions in which viral DNA was more frequently detected than viral antigen. This suggests that viral DNA may play a role in basal cell stimulation. Moreover some of these lesions with dense inflammatory reactions showed DR antigen expression by epithelial cells. Our findings indicate that epithelial cell activation in papillomas might be modulated by other factors than HPV such as mediators of the local immune response.
通过对35例不消退的皮肤和黏膜人乳头瘤进行冷冻切片间接免疫荧光检查，研究其表皮生长因子（EGF）和转铁蛋白受体的表达情况。还通过用生物素化探针和病毒衣壳抗原进行原位杂交，检查病变中是否存在人乳头瘤病毒（HPV）DNA。在具有严重病毒细胞病变效应的皮肤病变中，EGF和转铁蛋白受体的定位发生改变，而在主要为喉乳头瘤的黏膜病变中增强，喉乳头瘤病毒产生较少。在黏膜病变组中观察到EGF和转铁蛋白受体反应性增加最为显著，其中病毒DNA的检测频率高于病毒抗原。这表明病毒DNA可能在基底细胞刺激中起作用。此外，一些有密集炎症反应的病变显示上皮细胞表达DR抗原。我们的研究结果表明，乳头瘤中的上皮细胞活化可能受HPV以外的其他因素调节，如局部免疫反应的介质。
