On the similarity of triacylglycerol and acylcholesterol lipases in rat brain.

Previously, we described a similar stimulating effect of adrenocorticotrophic hormone (ACTH) and ACTH-related synthetic peptides on triacylglycerol (TAGL) and acylcholesterol (ACL) lipase activities of rat brain. In present study, TAGL and ACL activities from rat brain were further investigated and compared through the use of tri-[3H]oleoylglycerol and [14C]oleoylcholesterol, respectively, as the substrates. A number of specific properties proved to be similar for both activities: (a) comparable rates of activity decay were observed upon heat treatment, (b) similar reaction kinetics as determined at different substrate concentrations with and without ACTH (10(-4) M), (c) both activities showed a common susceptibility to the presence of sodium taurocholate (5 mM) or trace amounts (5 microliter/ml) of organic solvent in the assay medium, (d) the distributions of the two activities in various brain regions were comparable. Taken together, these data, along with the similar activability by ACTH, suggest a common structural basis for TAGL and ACL activities in rat brain.