Rose M, Botstein D
J Mol Biol. 1983 Nov 15;170(4):883-904. doi: 10.1016/s0022-2836(83)80193-4.
Expression of the URA3 gene of Saccharomyces cerevisiae was studied by analysis of URA3-lacZ gene fusions constructed in vitro. Synthesis of hybrid beta-galactosidase by fusions in frame with the coding sequence for orotidine-5'-phosphate decarboxylase (OMPdecarboxylase) was found to be normally regulated even when only 11 nucleotides of URA3 coding sequence remained, indicating that all transcription initiation and regulatory sites are present at the beginning of the URA3 gene. An upstream initiator codon that begins a short overlapping coding sequence in another reading frame was also found to be active in producing hybrid beta-galactosidase. However this beta-galactosidase synthesis showed little or no regulation. Nuclease protection experiments revealed numerous species of URA3 mRNA. The regulation of these is consistent with the idea that the URA3 protein and the overlapping peptide are translated from differentially regulated mRNAs of different lengths.
通过对体外构建的URA3 - lacZ基因融合体进行分析，研究了酿酒酵母URA3基因的表达。发现与乳清苷 - 5'-磷酸脱羧酶（OMP脱羧酶）编码序列框内融合产生的杂交β-半乳糖苷酶的合成即使在URA3编码序列仅剩下11个核苷酸时仍受到正常调节，这表明所有转录起始和调控位点都存在于URA3基因的起始处。还发现一个上游起始密码子，它在另一个阅读框中起始一个短的重叠编码序列，在产生杂交β-半乳糖苷酶时也具有活性。然而，这种β-半乳糖苷酶的合成几乎没有或没有调节作用。核酸酶保护实验揭示了多种URA3 mRNA。这些mRNA的调节与URA3蛋白和重叠肽从不同长度的差异调节mRNA翻译而来的观点一致。
