Ty-lacZ融合蛋白在酿酒酵母中的表达。
Expression of Ty-lacZ fusions in Saccharomyces cerevisiae.
作者信息
Bowen B A, Fulton A M, Tuite M F, Kingsman S M, Kingsman A J
出版信息
Nucleic Acids Res. 1984 Feb 10;12(3):1627-40. doi: 10.1093/nar/12.3.1627.
Abstract
We have determined the nucleotide sequence of about 520 bp spanning the 5' delta regions (Figure 1) of two Tyl elements. There is an open reading frame running out of the deltas for at least 180 nucleotides into the internal region of each element. The functional significance of these open reading frames has been tested by fusing them to a defective E.coli lacZ gene. Expression of B-galactosidase in yeast transformants containing these fusions shows that Tyl elements contain functional translation signals.
摘要
我们已经确定了跨越两个Tyl元件5'δ区域（图1）的约520bp的核苷酸序列。有一个开放阅读框从δ区域延伸到每个元件内部区域至少180个核苷酸。通过将这些开放阅读框与缺陷型大肠杆菌lacZ基因融合，测试了它们的功能意义。在含有这些融合体的酵母转化体中β-半乳糖苷酶的表达表明Tyl元件含有功能性翻译信号。
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