大肠杆菌素Ib质粒基因对T7噬菌体复制的抑制作用。

Inhibition of T7 bacteriophage replication by a colicin Ib plasmid gene.

作者信息

Duckworth D H, Garrity R R, McCorquodale D J, Pinkerton T C

出版信息

Virology. 1983 Nov;131(1):259-63. doi: 10.1016/0042-6822(83)90552-4.

Abstract

Clones containing fragments of the colicin Ib (ColIb) plasmid inserted into pBR322 have been found that inhibit the replication of T7 bacteriophage. Cells containing the whole ColIb plasmid grow T7 normally but cannot grow T7 protein kinase-negative mutants of T7. The cloned fragments inhibit not only the T7 protein kinaseless mutants but wild-type T7 as well. However, the whole plasmid can suppress the wild-type T7 inhibition caused by the cloned inhibiting genes. These results are consistent with a model in which a ColIb gene (pic) exists which can inhibit replication of T7 phage. A second gene (rpi) can repress the function of pic provided the rpi product is phosphorylated.

摘要

已发现含有插入pBR322的大肠杆菌素Ib（ColIb）质粒片段的克隆能抑制T7噬菌体的复制。含有完整ColIb质粒的细胞能正常培养T7，但不能培养T7蛋白激酶阴性的T7突变体。克隆片段不仅抑制T7无蛋白激酶突变体，也抑制野生型T7。然而，完整质粒可抑制由克隆的抑制基因引起的野生型T7抑制。这些结果与一种模型一致，即存在一个可抑制T7噬菌体复制的ColIb基因（pic）。如果rpi产物被磷酸化，第二个基因（rpi）可抑制pic的功能。