Pinkerton T, Muzyczka N, Walia S, Dunn G, Rose K, Duckworth D
Gene. 1981 Dec;16(1-3):89-96. doi: 10.1016/0378-1119(81)90064-0.
PstI restriction fragments of the colicin Ib (ColIb) plasmid have been cloned into the Apr gene of the pBR322 vector. Colicin-producing clones (Col+) all contained two common PstI (L and U) fragments, 2000 and 800 bp long, respectively. All of these colicin producers were found to permit the normal growth of bacteriophage T5; the presence of the whole ColIb plasmid causes an abortive T5 or BF23 phage infection. None of the other clones selected for their inability to propagate T5 produced colicin. The clones (Abi+) that permitted only an abortive infection by T5 contained a single restriction fragment, O (1600 bp), which was not found in any of the colicin producers. Likewise, the specific fragments (L and U) found in the Col+ clones were not found in the Abi+ clones. These data are very hard to reconcile with the hypothesis of a colicin-induced cell deterioration after T5 phage infection.
大肠杆菌素Ib（ColIb）质粒的PstI限制性片段已被克隆到pBR322载体的Apr基因中。产生大肠杆菌素的克隆（Col+）都含有两个常见的PstI片段（L和U），长度分别为2000和800 bp。所有这些产生大肠杆菌素的克隆都被发现能使噬菌体T5正常生长；完整的ColIb质粒的存在会导致T5或BF23噬菌体感染失败。其他因不能繁殖T5而被挑选出的克隆均不产生大肠杆菌素。那些只允许T5进行流产感染的克隆（Abi+）含有一个单一的限制性片段O（1600 bp），该片段在任何产生大肠杆菌素的克隆中都未发现。同样，在Col+克隆中发现的特定片段（L和U）在Abi+克隆中也未发现。这些数据很难与T5噬菌体感染后大肠杆菌素诱导细胞恶化的假说相吻合。
