Thornton G B, Kopchick J J, Stacey D W, Banerjee A K
Biochem Biophys Res Commun. 1983 Nov 15;116(3):1160-7. doi: 10.1016/s0006-291x(83)80264-2.
Microinjection of purified transcriptionally active ribonucleoprotein (RNP) complex of vesicular stomatitis virus in vero cells resulted in the production and release of virus. Compared to the release of virus by cells treated with RNP in the presence of DEAE-dextran, the microinjection technique was highly efficient. Microinjection in Xenopus oocytes also resulted in initiation of infection as shown by the synthesis of virus-specific proteins in the cell cytoplasm. It was further observed that RNP stripped of L protein but containing residual NS protein was capable of initiating virus production or protein synthesis when microinjected in vero cells or in oocytes, respectively. Since L and NS proteins are essential for in vitro transcription by RNP, these results suggest that a trace amount of L protein may remain bound to the RNP and a host factor may stimulate residual L activity in vivo.
将水疱性口炎病毒纯化的具有转录活性的核糖核蛋白（RNP）复合物显微注射到非洲绿猴肾细胞中，可导致病毒的产生和释放。与在存在二乙氨基乙基葡聚糖的情况下用RNP处理的细胞释放病毒相比，显微注射技术效率很高。在非洲爪蟾卵母细胞中进行显微注射也导致感染的启动，如细胞质中病毒特异性蛋白的合成所示。进一步观察到，去除L蛋白但含有残留NS蛋白的RNP，分别显微注射到非洲绿猴肾细胞或卵母细胞中时，能够启动病毒产生或蛋白质合成。由于L蛋白和NS蛋白对于RNP的体外转录至关重要，这些结果表明，可能有微量的L蛋白仍与RNP结合，并且一种宿主因子可能在体内刺激残留的L蛋白活性。
