Carroll A R, Wagner R R
J Virol. 1978 Feb;25(2):675-84. doi: 10.1128/JVI.25.2.675-684.1978.
Specific antisera were raised by immunization of rabbits with purified nucleocapsids containing only RNA and N protein (ribonucleoprotein, RNP) obtained from vesicular stomatitis (VS) virions of the Indiana (VSInd) and the New Jersey (VSNJ) serotypes. The specificity of anti-RNPInd serum was demonstrated by selective precipitation of homotypic RNPInd devoid of L and NS proteins; anti-RNPInd serum also selectively precipitated soluble N protein present in cytoplasm of infected cells, but co-precipitated a limited amount of contaminating soluble NS protein. Immunoglobulins prepared from each homotypic antiserum markedly inhibited in vitro transcription of VSInd and VSNJ virions. Anti-RNPInd and anti-RNPNJ immunoglobulins also exhibited cross-reactivity by inhibiting transcription of heterotypic virions, but only to a much lesser degree than in the homotypic reaction. Anti-RNPInd immunoglobulin did not inhibit transcription of the antigenically unrelated Chandipura rhabdovirus, but anti-RNPNJ immunoglobulin did to a very limited extent. The transcription inhibitory activity of anti-RNPInd immunoglobulin was not dependent on RNP immunoprecipitation activity, which could be diluted out well before loss of antitranscriptase activity. Anti-RNPind immunoglobulin appeared to exert its effect on transcription by blocking elongation rather than initiation or reinitiation of RNA transcripts.
通过用从印第安纳州（VSInd）和新泽西州（VSNJ）血清型的水疱性口炎（VS）病毒粒子中获得的仅含有RNA和N蛋白（核糖核蛋白，RNP）的纯化核衣壳免疫兔子，制备了特异性抗血清。抗RNPInd血清的特异性通过选择性沉淀不含L和NS蛋白的同型RNPInd来证明；抗RNPInd血清还选择性沉淀感染细胞胞质中存在的可溶性N蛋白，但共沉淀了少量污染的可溶性NS蛋白。从每种同型抗血清制备的免疫球蛋白显著抑制VSInd和VSNJ病毒粒子的体外转录。抗RNPInd和抗RNPNJ免疫球蛋白也通过抑制异型病毒粒子的转录表现出交叉反应性，但程度远低于同型反应。抗RNPInd免疫球蛋白不抑制抗原无关的钱迪普拉弹状病毒的转录，但抗RNPNJ免疫球蛋白在非常有限的程度上有抑制作用。抗RNPInd免疫球蛋白的转录抑制活性不依赖于RNP免疫沉淀活性，在抗转录酶活性丧失之前，RNP免疫沉淀活性可以很好地被稀释掉。抗RNPind免疫球蛋白似乎通过阻断RNA转录物的延伸而不是起始或重新起始来发挥其对转录的作用。
