Kikuchi Y
Cell. 1983 Dec;35(2 Pt 1):487-93. doi: 10.1016/0092-8674(83)90182-4.
Unstable ars1 (autonomously replicating sequence)-containing plasmids can be stabilized by connecting particular DNA segments of yeast 2 mu-plasmid. A cis-acting locus for the plasmid's stability (the STB locus) was found in the large unique region and separated from the replication origin of 2 mu-plasmid. Several direct repeats found in this region were essential for its function. Two proteins encoded by the plasmid (B and C) were required as trans-acting factors. The average copy number of the plasmid was not affected whether the plasmid carried the stability system or not, suggesting that the system was not directly associated with the replication function but was involved in partitioning at cell division.
含有不稳定自主复制序列（ars1）的质粒可通过连接酵母2μm质粒的特定DNA片段来实现稳定。在大的单一区域中发现了一个对质粒稳定性起顺式作用的位点（STB位点），它与2μm质粒的复制起点分开。该区域中发现的几个直接重复序列对其功能至关重要。质粒编码的两种蛋白质（B和C）作为反式作用因子是必需的。无论质粒是否携带稳定性系统，其平均拷贝数均不受影响，这表明该系统与复制功能没有直接关联，而是参与细胞分裂时的分配过程。
