Adam G, Steiner U, Seuwen K
Cell Biol Int Rep. 1983 Nov;7(11):955-62. doi: 10.1016/0309-1651(83)90215-1.
The phenomenon of resistance of confluent 3T3 cells to serum stimulation of proliferation is investigated and shown to depend drastically on the kinetics of application of growth factors with the serum. The cellular content of ribosomal RNA is determined flow-cytometrically by measuring the red fluorescence of cells stained with acridine orange. Ribosomal RNA content of 3T3 cells, but not of SV40-3T3 cells, is shown to decrease after confluence. The kinetics of restoration of ribosomal RNA in stimulated 3T3 cells exhibits an increasing delay with increasing duration of quiescence. These and further results are consistent with "progression" to initiation of the cell cycle being closely associated with restoration of cellular content of ribosomes.
研究了汇合的3T3细胞对血清刺激增殖的抗性现象，并表明其极大地依赖于生长因子与血清应用的动力学。通过测量用吖啶橙染色的细胞的红色荧光，流式细胞术测定核糖体RNA的细胞含量。结果显示，汇合后3T3细胞而非SV40-3T3细胞的核糖体RNA含量降低。在受刺激的3T3细胞中，核糖体RNA恢复的动力学表现出随着静止持续时间的增加而延迟增加。这些以及其他结果与细胞周期起始的“进展”与核糖体细胞含量的恢复密切相关是一致的。
