Wood J N
Mol Cell Biochem. 1979 Jul 15;26(1):29-32. doi: 10.1007/BF00226818.
Rapidly labelled nuclear RNA from an SV40 transformed mouse 3T3 line was isolated, and different molecular size classes pooled. The fractions were treated with Ehrlich ascites exonuclease III for various lengths of time, and the digestion of the 3' ends of the RNA measured by the appearance of TCA-soluble material. The resulting partially degraded RNA populations were then hybridised to SV40 DNA. The data suggest that a high proportion of the viral specific sequences are located near the 3' ends of heterogeneous nuclear RNA.
从SV40转化的小鼠3T3细胞系中分离出快速标记的核RNA，并将不同分子大小的类别合并。将这些组分用艾氏腹水外切核酸酶III处理不同时间，并通过TCA可溶性物质的出现来测量RNA 3'端的消化情况。然后将产生的部分降解的RNA群体与SV40 DNA杂交。数据表明，很大一部分病毒特异性序列位于异质核RNA的3'端附近。
