Sharif N A, Burt D R, Towle A C, Mueller R A, Breese G R
Eur J Pharmacol. 1983 Nov 25;95(3-4):301-4. doi: 10.1016/0014-2999(83)90651-9.
Thyrotropin-releasing hormone (TRH), substance P (SP) and serotonin (5-HT) coexist in raphe efferents to the spinal cord. Lesions of these serotonergic projections by intracisternal injection of 5,7-dihydroxytryptamine (50 micrograms) in rat pups resulted in 93% and 65% decreases in 5-HT and TRH content of adult rat spinal cords, respectively. This codepletion induced a 30-40% increase in binding of [3H][3-Me-His2]TRH [( 3H]MeTRH) to TRH receptors. The affinity and pharmacological specificity of [3H]MeTRH binding to control and DHT-lesioned cords appeared closely similar.
促甲状腺激素释放激素（TRH）、P物质（SP）和5-羟色胺（5-HT）共存于中缝脊髓传出纤维中。在新生大鼠脑池中注射5,7-二羟色胺（50微克）损毁这些5-羟色胺能投射，分别导致成年大鼠脊髓中5-HT和TRH含量降低93%和65%。这种共同耗竭使[3H][3-甲基组氨酸2]TRH（[3H]MeTRH）与TRH受体的结合增加30%-40%。[3H]MeTRH与对照脊髓和5,7-二羟色胺损毁脊髓结合的亲和力和药理学特异性似乎非常相似。
