5-羟色胺和促甲状腺激素释放激素共同耗竭后大鼠脊髓中促甲状腺激素释放激素（TRH）受体的上调

Up-regulation of thyrotropin-releasing hormone (TRH) receptors in rat spinal cord after codepletion of serotonin and TRH.

作者信息

Ogawa N, Kabuto H, Hirose Y, Nukina I, Mori A

出版信息

Regul Pept. 1985 Mar;10(2-3):85-90. doi: 10.1016/0167-0115(85)90002-3.

Abstract

Immunohistochemical evidence indicates the coexistence of serotonin and TRH in many raphe neurons. We examined the biochemical changes in TRH receptors after destruction of the serotonergic pathways by 5,7-dihydroxytryptamine (5,7-DHT). 2 weeks after an intracerebroventricular injection of 5,7-DHT, rats were killed, and specific brain regions were dissected on ice. Serotonin levels in the CNS of lesioned rats was reduced by 50-85% in all regions, with the highest reduction in the spinal cord and hippocampus. Immunoreactive TRH was reduced in the spinal cord by 70%, but other brain regions contained normal levels of TRH. TRH receptor binding was increased by 40% in the spinal cord of lesioned rats, but appeared unchanged in rostral brain regions in which no decrease in TRH content was detected. Scatchard plots of TRH receptor binding in the spinal cord indicated that the increased binding after 5,7-DHT administration reflected an increased receptor number. These findings suggest that biochemical up-regulation of TRH receptors occurs in the spinal cord following depletion of TRH.

摘要

免疫组织化学证据表明，5-羟色胺和促甲状腺激素释放激素（TRH）在许多中缝神经元中共存。我们研究了用5,7-二羟基色胺（5,7-DHT）破坏血清素能通路后TRH受体的生化变化。脑室内注射5,7-DHT两周后，处死大鼠，并在冰上解剖特定脑区。损伤大鼠中枢神经系统中所有区域的血清素水平均降低了50-85%，脊髓和海马体中的降低幅度最大。脊髓中免疫反应性TRH降低了70%，但其他脑区的TRH水平正常。损伤大鼠脊髓中TRH受体结合增加了40%，但在未检测到TRH含量降低的脑桥脑区中似乎没有变化。脊髓中TRH受体结合的Scatchard图表明，5,7-DHT给药后结合增加反映了受体数量增加。这些发现表明，TRH耗竭后脊髓中会发生TRH受体的生化上调。