Kopeikina-Tsiboukidou L, Deliconstantinos G
Int J Biochem. 1983;15(12):1403-7. doi: 10.1016/0020-711x(83)90071-x.
The binding of [14C]cholesterol into rat brain mitochondrial membranes follows an exponential path described by the general formula y = a X ebx. [14C]cholesterol glucoside binding has a sigmoidal character where the "best-fit" curve of this type of binding is the one described by the Hill equation with Hill coefficient h = 2.06. These findings suggest a positive cooperativity in the binding of both compounds into rat brain mitochondrial membranes. The specific activity of the outer mitochondrial membrane enzyme monoamine oxidase was linearly decreased at different concentration of cholesterol or its glucoside. The specific activity of the inner mitochondrial membrane enzyme succinate-cytochrome c reductase was linearly decreased, while that of Rotenone-sensitive NADH-cytochrome c reductase was exponentially increased, at different concentrations of cholesterol. These results are discussed in terms of specific interactions of cholesterol with constituent mitochondrial membrane lipids and their implications for deviations from normal neuronal function.
[14C]胆固醇与大鼠脑线粒体膜的结合遵循由通式y = a×ebx描述的指数路径。[14C]胆固醇葡糖苷的结合具有S形特征，这种结合类型的“最佳拟合”曲线是由希尔方程描述的曲线，希尔系数h = 2.06。这些发现表明这两种化合物与大鼠脑线粒体膜的结合存在正协同性。线粒体外膜单胺氧化酶的比活性在不同浓度的胆固醇或其葡糖苷作用下呈线性下降。线粒体内膜琥珀酸 - 细胞色素c还原酶的比活性呈线性下降，而鱼藤酮敏感的NADH - 细胞色素c还原酶的比活性在不同浓度的胆固醇作用下呈指数增加。本文根据胆固醇与线粒体膜脂质成分的特异性相互作用及其对正常神经元功能偏差的影响对这些结果进行了讨论。
