用于在大肠杆菌和灰色链霉菌C581中克隆重组DNA的穿梭载体。
Shuttle vectors for cloning recombinant DNA in Escherichia coli and Streptomyces griseofuscus C581.
作者信息
Larson J L, Hershberger C L
出版信息
J Bacteriol. 1984 Jan;157(1):314-7. doi: 10.1128/jb.157.1.314-317.1984.
Abstract
The replicon of the Streptomyces plasmid SCP2 was located on a 5.9-kilobase EcoRI-SalI restriction fragment. The SCP2 replicon was combined with Escherichia coli plasmid pBR322 and genes specifying neomycin resistance and thiostrepton resistance in streptomycetes to construct shuttle vectors that are useful for cloning in E. coli and streptomycetes.
摘要
链霉菌质粒SCP2的复制子位于一个5.9千碱基的EcoRI-SalI限制性片段上。将SCP2复制子与大肠杆菌质粒pBR322以及链霉菌中指定新霉素抗性和硫链丝菌素抗性的基因相结合，构建了可用于在大肠杆菌和链霉菌中克隆的穿梭载体。
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本文引用的文献
1
A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces.一种用于抗生素产生菌链霉菌种间基因转移的DNA克隆系统。
Nature. 1980 Apr 10;284(5756):526-31. doi: 10.1038/284526a0.
2
Protoplast fusion in Streptomyces: conditions for efficient genetic recombination and cell regeneration.链霉菌中的原生质体融合：高效基因重组和细胞再生的条件
J Gen Microbiol. 1981 Nov;127(1):137-46. doi: 10.1099/00221287-127-1-137.
3
Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans.从天蓝色链霉菌中切除染色体DNA序列可形成一个能在淡紫灰链霉菌中检测到的新型质粒家族。
Mol Gen Genet. 1981;184(2):230-40. doi: 10.1007/BF00272910.
4
Amplified DNA in Streptomyces fradiae.弗氏链霉菌中的扩增DNA。
J Bacteriol. 1983 Aug;155(2):459-66. doi: 10.1128/jb.155.2.459-466.1983.
5
Development of cloning vehicles from the Streptomyces plasmid pFJ103.基于链霉菌质粒pFJ103构建克隆载体
Gene. 1982 Dec;20(3):451-7. doi: 10.1016/0378-1119(82)90214-1.
6
Physical analysis of antibiotic-resistance genes from Streptomyces and their use in vector construction.链霉菌抗生素抗性基因的物理分析及其在载体构建中的应用。
Gene. 1982 Nov;20(1):51-62. doi: 10.1016/0378-1119(82)90086-5.
7
[Cloning the DNA fragments of Streptomyces coelicolor A3(2) plasmid SCP2 in Escherichia coli cells].[在大肠杆菌细胞中克隆天蓝色链霉菌A3(2) 质粒SCP2的DNA片段]
Genetika. 1982 Nov;18(11):1811-6.
8
Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
Nucleic Acids Res. 1982 Mar 11;10(5):r117-44. doi: 10.1093/nar/10.5.1770.
9
Gene cloning in Streptomyces.链霉菌中的基因克隆
Curr Top Microbiol Immunol. 1982;96:69-95. doi: 10.1007/978-3-642-68315-2_5.
10
DNA cloning in Streptomyces: resistance genes from antibiotic-producing species.链霉菌中的DNA克隆：来自抗生素产生菌的抗性基因。
Nature. 1980 Jul 31;286(5772):525-7. doi: 10.1038/286525a0.
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