呼吸道合胞病毒在活跃生长的人喉表皮样癌细胞（HEp-2细胞）中的复制情况改善。

Improvement of respiratory syncytial virus replication in actively growing HEp-2 cells.

作者信息

Pons M W, Lambert A L, Lambert D M, Rochovansky O M

出版信息

J Virol Methods. 1983 Oct;7(4):217-21. doi: 10.1016/0166-0934(83)90011-3.

Abstract

The growth of respiratory syncytial (RS) and parainfluenza type III (PI3) viruses has been studied in actively growing versus relatively stationary HEp-2 cells. There was no effect on PI3 virus growth. RS virus synthesis was stimulated from 20- to 60-fold by growth in actively growing cultures when the cell density was approximately 1/3 that of a confluent culture. This stimulation was manifested by a greater yield of virus per cell, more virus-specific mRNA produced per cell, and a 50% decrease in the time before cytopathic changes appeared.

摘要

在生长活跃的与相对静止的人喉表皮样癌细胞（HEp-2）中，对呼吸道合胞体（RS）病毒和III型副流感病毒（PI3）的生长情况进行了研究。这对PI3病毒的生长没有影响。当细胞密度约为铺满培养物密度的1/3时，在生长活跃的培养物中生长可使RS病毒的合成增加20至60倍。这种促进作用表现为每个细胞产生的病毒产量更高、每个细胞产生的病毒特异性信使核糖核酸（mRNA）更多，以及出现细胞病变变化前的时间减少50%。