Mensing H, Czarnetzki B M
J Invest Dermatol. 1984 Jan;82(1):9-12. doi: 10.1111/1523-1747.ep12258678.
In vitro fibroblast chemotaxis was studied in the presence of synthetic leukotriene B4 (LTB4) and of supernatants from ionophore-stimulated lymphocytes, monocytes, and basophils (LMB-S). LTB4 and LMB-S induced a dose-related, directed migration of human and rat embryonic fibroblasts. By checkerboard analysis, this response was chemotactic and not chemokinetic. Optimal migration toward LTB4 occurred at 10(-8) M concentrations, while higher concentrations were inhibitory. The LMB-S contained two types of fibroblast chemotactic factors, one that corresponded to LTB4 and one additional, heat labile, non-dialyzable substance of greater than 10 kilodaltons. LTB4 and LMB-S were inactive against transformed human connective tissue cells (HT 1080, McCoy) while these cells moved well in response to conditioned medium derived from confluent fibroblast monolayer cultures. These results suggest that LTB4 is a specific chemoattractant for fibroblasts and that it acts in concert with other chemotactic factors derived from inflammatory leukocytes to regulate the influx of fibroblasts to tissue sites.
在合成白三烯B4（LTB4）以及离子载体刺激的淋巴细胞、单核细胞和嗜碱性粒细胞的上清液（LMB - S）存在的情况下，研究了体外成纤维细胞趋化性。LTB4和LMB - S诱导了人及大鼠胚胎成纤维细胞的剂量相关的定向迁移。通过棋盘分析，这种反应是趋化性的而非趋动性的。对LTB4的最佳迁移发生在10^(-8) M浓度时，而更高浓度则具有抑制作用。LMB - S含有两种成纤维细胞趋化因子，一种与LTB4相对应，另一种是额外的、热不稳定的、大于10千道尔顿的不可透析物质。LTB4和LMB - S对转化的人结缔组织细胞（HT 1080， McCoy）无活性，而这些细胞对来自汇合的成纤维细胞单层培养物的条件培养基反应良好。这些结果表明，LTB4是成纤维细胞的一种特异性趋化因子，并且它与源自炎性白细胞的其他趋化因子协同作用，以调节成纤维细胞向组织部位的流入。
