人中性粒细胞和淋巴细胞/单核细胞体外产生趋化活性白三烯B4及其ω-代谢产物的比较。
Comparison of the generation in vitro of chemotactically active LTB4 and its omega-metabolites by human neutrophils and lymphocytes/monocytes.
作者信息
Rosenbach T, Czarnetzki B M
机构信息
University Clinics, Department of Dermatology, Münster, FRG.
出版信息
Clin Exp Immunol. 1987 Jul;69(1):221-8.
To assess the relative contribution of different leucocyte subpopulations to LTB4 production, peripheral blood leucocytes from human donors were separated into polymorphonuclear neutrophils (PMN) and lymphocytes/monocytes (L/M) and were then stimulated in vitro with the Ca-ionophore A 23187 for different times. The supernatants were analysed for their contents of leukotriene B4 (LTB4) and its omega-metabolites by HPLC-analysis and column fractions were also examined for their chemotactic activities towards eosinophils in vitro. PMN supernatants contained greater quantities of LTB4, 20-OH-LTB4, 20-COOH-LTB4, and chemotactic activities than did L/M supernatants. On the other hand, the time dependent decrease of LTB4 and chemotactic activity and the increase of omega-metabolites were higher in PMN than in L/M. These results would correlate with the greater role of PMN in acute and that of monocytes in chronic inflammation.
为评估不同白细胞亚群对白三烯B4（LTB4）产生的相对贡献，将人类供体的外周血白细胞分离为多形核中性粒细胞（PMN）和淋巴细胞/单核细胞（L/M），然后用钙离子载体A 23187在体外刺激不同时间。通过高效液相色谱分析（HPLC分析）检测上清液中白三烯B4（LTB4）及其ω-代谢产物的含量，并检测柱馏分对嗜酸性粒细胞的体外趋化活性。PMN上清液中LTB4、20-羟基-LTB4、20-羧基-LTB4的含量以及趋化活性均高于L/M上清液。另一方面，PMN中LTB4和趋化活性的时间依赖性降低以及ω-代谢产物的增加高于L/M。这些结果与PMN在急性炎症中起更大作用以及单核细胞在慢性炎症中起更大作用相关。
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