Shiraishi Y, Tanaka Y, Kato M, Miwa M, Sugimura T
Mutat Res. 1983 Nov;122(2):223-8. doi: 10.1016/0165-7992(83)90063-5.
The effect of inhibitors of poly(ADP-ribose)polymerase, benzamide (Bam) and m-aminobenzamide (m-AB), on sister-chromatid exchanges (SCEs) and cell growth, was examined in lymphoblastoid cell lines from a normal adult (KS-64) and from a Bloom syndrome patient (BS1-2). The presence of Bam and m-AB increased the levels of SCEs in KS-64 and BS1-2 lymphoblastoid cells. Though the net increase was similar in the two types of cell, the relative increase was much lower in the BS1-2 cells. Bam and m-AB increased the number of SCEs in BS1-2 cells to levels of 95.4 +/- 3.24 and 98.1 +/- 3.23 per cell, respectively, as compared with the baseline level of 75.5 +/- 2.16. On the other hand, when KS-64 cells were treated with Bam and m-AB, the number of SCEs increased to 27.1 +/- 1.98 and 28.6 +/- 2.71 per cell, respectively, compared with the baseline number of 6.7 +/- 0.41 per cell. These inhibitors of poly(ADP-ribose)polymerase also inhibited cell growth at concentrations which induced SCEs in KS-64 as well as in BS1-2 cells. No significant decrease in the poly(ADP-ribose)polymerase activity or in the amount of poly-(ADP-ribose) was detected in BS1-2 cells as compared with KS-64 cells. The mechanism by which SCEs are increased in BS1-2 cells is discussed.
研究了聚（ADP - 核糖）聚合酶抑制剂苯甲酰胺（Bam）和间氨基苯甲酰胺（m - AB）对来自正常成年人的淋巴母细胞系（KS - 64）和来自布卢姆综合征患者的淋巴母细胞系（BS1 - 2）中姐妹染色单体交换（SCEs）及细胞生长的影响。Bam和m - AB的存在增加了KS - 64和BS1 - 2淋巴母细胞中的SCEs水平。尽管两种细胞类型中的净增加相似，但BS1 - 2细胞中的相对增加要低得多。与基线水平75.5±2.16相比，Bam和m - AB分别将BS1 - 2细胞中的SCEs数量增加到每细胞95.4±3.24和98.1±3.23。另一方面，用Bam和m - AB处理KS - 64细胞时，与每细胞基线数量6.7±0.41相比，SCEs数量分别增加到每细胞27.1±1.98和28.6±2.71。这些聚（ADP - 核糖）聚合酶抑制剂在诱导KS - 64和BS1 - 2细胞发生SCEs的浓度下也抑制细胞生长。与KS - 64细胞相比，未检测到BS1 - 2细胞中聚（ADP - 核糖）聚合酶活性或聚（ADP - 核糖）量有显著降低。本文讨论了BS1 - 2细胞中SCEs增加的机制。
