3-氨基苯甲酰胺和紫外线对HeLa细胞姐妹染色单体交换的独立诱导作用。
Independent induction of sister-chromatid exchanges by 3-aminobenzamide and ultraviolet radiation in HeLa cells.
作者信息
Otsuka F, Ohno H, Enomoto T, Hanaoka F, Yamada M
出版信息
Mutat Res. 1983 Feb;107(2):289-96. doi: 10.1016/0027-5107(83)90170-7.
PMID:6408468
Abstract
The effect of 3-aminobenzamide in HeLa cells was examined in several aspects. 10(-3) M 3-aminobenzamide inhibited poly(ADP-ribose) polymerase activity to more than 95% in disrupted nuclei of HeLa cells. The mode of inhibition of the enzyme was competitive inhibition with NAD at a Ki value of 2.5 microM 3-aminobenzamide. The combined treatment of HeLa cells with 3-aminobenzamide and ultraviolet radiation revealed the independent induction of SCEs by these 2 agents.
摘要
从多个方面研究了3-氨基苯甲酰胺对HeLa细胞的作用。10⁻³ M的3-氨基苯甲酰胺可使HeLa细胞破碎核中的聚（ADP-核糖）聚合酶活性抑制超过95%。该酶的抑制模式为与NAD的竞争性抑制，3-氨基苯甲酰胺的Ki值为2.5 μM。用3-氨基苯甲酰胺和紫外线联合处理HeLa细胞，发现这两种试剂可独立诱导姐妹染色单体交换。
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