Quirion R, Gaudreau P, St-Pierre S, Rioux F
Peptides. 1982 Sep-Oct;3(5):765-9. doi: 10.1016/0196-9781(82)90012-2.
[3H]Neurotensin ([3H]NT) appears to bind specifically to a single class of sites in slide-mounted rat kidney sections (KD = 8.3 nM; Bmax = 31.6 fmol/mg tissue). Bound [3H]NT can be displaced by nonradioactive NT and a series of its fragments and analogues with relative potencies that correlate well (r = 0.91; p less than 0.005) to their potencies in the rat stomach strip bioassay. These results suggest that NT receptors are similar in both systems. However, they are probably slightly different from those present in the guinea pig atria (r = 0.78; p less than 0.1). We visualized these sites by using the tritium-sensitive LKB film technique analysed by computerized densitometry. [3H]NT binding sites are highly concentrated in the renal cortex while low levels are observed in the renal medulla. The possible physiological and/or pathophysiological significances of the presence of [3H]NT binding sites in the kidney are discussed.
[3H]神经降压素（[3H]NT）似乎能特异性结合载玻片上大鼠肾脏切片中的一类单一位点（解离常数KD = 8.3纳摩尔；最大结合容量Bmax = 31.6飞摩尔/毫克组织）。结合的[3H]NT可被非放射性NT及其一系列片段和类似物取代，其相对效力与它们在大鼠胃条生物测定中的效力具有良好的相关性（r = 0.91；p < 0.005）。这些结果表明，两种系统中的神经降压素受体相似。然而，它们可能与豚鼠心房中的受体略有不同（r = 0.78；p < 0.1）。我们使用经计算机密度测定分析的对氚敏感的LKB胶片技术对这些位点进行了可视化。[3H]NT结合位点在肾皮质中高度集中，而在肾髓质中水平较低。本文讨论了肾脏中存在[3H]NT结合位点可能具有的生理和/或病理生理意义。
