骨髓增殖性疾病中白细胞和血小板的花生四烯酸代谢改变。

Altered arachidonate metabolism by leukocytes and platelets in myeloproliferative disorders.

作者信息

Takayama H, Okuma M, Kanaji K, Sugiyama T, Sensaki S, Uchino H

出版信息

Prostaglandins Leukot Med. 1983 Nov;12(3):261-72. doi: 10.1016/0262-1746(83)90004-5.

Abstract

The arachidonate metabolism by leukocytes and platelets was studied in 14 patients with myeloproliferative disorders including 7 patients with chronic myeloid leukemia (CML), 5 with polycythemia vera (PV) and 2 with essential thrombocythemia (ET). When the leukocytes were incubated with arachidonate and A23187, leukotriene B4 and hydroxyeicosatetraenoic acids (HETEs) were constantly detected using reversed-phase high-performance liquid chromatography in normal subjects, while selective deficiency of 5-lipoxygenase products (leukotriene B4 and 5-HETE) was found in 4 patients with CML. this novel abnormality of the leukocytes seemed to be derived from the possible deficiency of 5-lipoxygenase in these patients' polymorphonuclear neutrophils (PMNNs). The formation of 15-HETE appeared to be almost normal in all the patients. Platelet 12-lipoxygenase deficiency was detected in 2 patients with PV and 2 with CML in whom one was associated with the deficiency of 5-lipoxygenase products. These bicellular abnormalities of the arachidonate metabolism might contribute to understand dysfunctions of PMNNs and platelets in some patients with myeloproliferative disorders.

摘要

对14例骨髓增殖性疾病患者的白细胞和血小板花生四烯酸代谢进行了研究，其中包括7例慢性粒细胞白血病（CML）患者、5例真性红细胞增多症（PV）患者和2例原发性血小板增多症（ET）患者。当白细胞与花生四烯酸和A23187一起孵育时，在正常受试者中使用反相高效液相色谱法可持续检测到白三烯B4和羟基二十碳四烯酸（HETEs），而在4例CML患者中发现了5-脂氧合酶产物（白三烯B4和5-HETE）的选择性缺乏。白细胞的这种新异常似乎源于这些患者多形核中性粒细胞（PMNNs）中5-脂氧合酶可能的缺乏。在所有患者中，15-HETE的形成似乎几乎正常。在2例PV患者和2例CML患者中检测到血小板12-脂氧合酶缺乏，其中1例与5-脂氧合酶产物缺乏有关。花生四烯酸代谢的这些双细胞异常可能有助于理解一些骨髓增殖性疾病患者中PMNNs和血小板的功能障碍。