Verbiese-Genard N, Hanocq M, Alvoet C, Molle L
Anal Biochem. 1983 Oct 1;134(1):170-5. doi: 10.1016/0003-2697(83)90279-8.
Optimal conditions for the extraction from brain tissue and the simultaneous quantification of catechol and indole derivatives were determined after a systematic degradation study in water and perchloric acid. The roles of three parameters, namely temperature, presence of antioxidant agents, and time, were considered. Adrenaline, noradrenaline, dopamine, homovanillic acid, 5-hydroxytryptophan, 5-hydroxyindole acetic acid, serotonin, and epinephrine were separated by HPLC and detected electrochemically. The results indicated a great instability of the indole derivatives at an ambient temperature, in an acid medium, and in the absence of a protective agent. Therefore, when perchloric acid has to be used for deproteinization, the lowest concentration (0.1 M) is preferable. The samples have to be kept on ice, in darkness, and protected by ascorbic acid and sodium ethylenediamine tetracetate.
在对水和高氯酸进行系统降解研究后，确定了从脑组织中提取并同时定量儿茶酚和吲哚衍生物的最佳条件。考虑了三个参数的作用，即温度、抗氧化剂的存在和时间。通过高效液相色谱法分离肾上腺素、去甲肾上腺素、多巴胺、高香草酸、5-羟色氨酸、5-羟吲哚乙酸、血清素和肾上腺素，并进行电化学检测。结果表明，吲哚衍生物在常温、酸性介质和无保护剂的情况下极不稳定。因此，当必须使用高氯酸进行脱蛋白时，最低浓度（0.1 M）是优选的。样品必须保存在冰上、暗处，并由抗坏血酸和乙二胺四乙酸钠保护。
