Effect of inhibitors of protein synthesis on dopamine modulation of the slow-EPSP in rabbit superior cervical ganglion.

The protein synthesis inhibitors, anisomycin and cycloheximide, were tested for their ability to prevent dopamine-induced long-term enhancement of the slow-EPSP in rabbit superior cervical ganglion. Exposure of ganglia to either inhibitor of protein synthesis, at a concentration that suppressed [3H]leucine incorporation into ganglionic protein by at least 95%, had no effect on the development of dopamine-induced enhancement of the slow-EPSP. Incubation of ganglia with dopamine, without an inhibitor of protein synthesis, was without effect on [3H]leucine incorporation into ganglionic protein. It is concluded that synthesis of new protein is not required for the development of long-term enhancement of the slow-EPSP induced by dopamine.