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多巴胺和去极化剂调节哺乳动物颈上神经节中蛋白I的磷酸化状态。

Dopamine and depolarizing agents regulate the state of phosphorylation of protein I in the mammalian superior cervical sympathetic ganglion.

作者信息

Nestler E J, Greengard P

出版信息

Proc Natl Acad Sci U S A. 1980 Dec;77(12):7479-83. doi: 10.1073/pnas.77.12.7479.

DOI:10.1073/pnas.77.12.7479
PMID:6164062
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC350528/
Abstract

The regulation of the state of phosphorylation of protein I, a specific neuronal protein that appears to be associated predominantly with synaptic vesicles, has been studied in intact sections of bovine superior cervical ganglion. For this purpose, a technique was developed that made possible the quantitation of the state of phosphorylation of as little as 5 fmol of protein I. Incubation of ganglion sections in the presence of dopamine, 8-bromo-cyclic AMP, or depolarizing agents (i.e., high K+ concentration or veratridine) increased the state of phosphorylation of protein I relative to that of control ganglion sections. Other results indicated that the effect of dopamine is probably mediated via the activation of a cyclic AMP-dependent protein kinase and that the effect of high K+ concentration is probably mediated via the activation of a calcium-dependent protein kinase.

摘要

蛋白I是一种特定的神经元蛋白，似乎主要与突触小泡相关，其磷酸化状态的调节已在牛颈上神经节的完整切片中进行了研究。为此，开发了一种技术，该技术能够对低至5飞摩尔的蛋白I的磷酸化状态进行定量。在多巴胺、8-溴环磷酸腺苷或去极化剂（即高钾浓度或藜芦碱）存在的情况下孵育神经节切片，相对于对照神经节切片，蛋白I的磷酸化状态增加。其他结果表明，多巴胺的作用可能是通过激活环磷酸腺苷依赖性蛋白激酶介导的，高钾浓度的作用可能是通过激活钙依赖性蛋白激酶介导的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a95b/350528/3161e0db9c13/pnas00499-0543-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a95b/350528/0cc4dd5bb4d7/pnas00499-0543-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a95b/350528/3161e0db9c13/pnas00499-0543-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a95b/350528/0cc4dd5bb4d7/pnas00499-0543-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a95b/350528/3161e0db9c13/pnas00499-0543-b.jpg

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Dopamine and depolarizing agents regulate the state of phosphorylation of protein I in the mammalian superior cervical sympathetic ganglion.多巴胺和去极化剂调节哺乳动物颈上神经节中蛋白I的磷酸化状态。
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本文引用的文献

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Opiate receptor agonists regulate phosphorylation of synapsin I in cocultures of rat spinal cord and dorsal root ganglion.阿片受体激动剂可调节大鼠脊髓和背根神经节共培养物中突触素I的磷酸化。
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Distribution of protein I in mammalian brain as determined by a detergent-based radioimmunoassay.采用基于去污剂的放射免疫分析法测定蛋白质I在哺乳动物脑中的分布。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2130-4. doi: 10.1073/pnas.78.4.2130.
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Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation.突触结合蛋白I（蛋白I），一种神经末梢特异性磷蛋白。III. 在高度纯化的突触小泡制剂中研究其与突触小泡的关联。
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Synapsin I (Protein I), a nerve terminal-specific phosphoprotein. II. Its specific association with synaptic vesicles demonstrated by immunocytochemistry in agarose-embedded synaptosomes.突触素I（蛋白I），一种神经末梢特异性磷蛋白。II. 通过免疫细胞化学在琼脂糖包埋的突触体中证明其与突触小泡的特异性结合。
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Synapsin I (protein I), a nerve terminal-specific phosphoprotein. I. Its general distribution in synapses of the central and peripheral nervous system demonstrated by immunofluorescence in frozen and plastic sections.突触素I（蛋白I），一种神经末梢特异性磷蛋白。I. 通过冷冻切片和塑料切片免疫荧光法显示其在中枢和外周神经系统突触中的总体分布。
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Generation of slow inhibitory and excitatory postsynaptic potentials.
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Effects of dopamine on the superior cervical ganglion of the rabbit.多巴胺对兔颈上神经节的作用。
J Physiol. 1974 May;239(1):155-64. doi: 10.1113/jphysiol.1974.sp010560.
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Immunocytochemical localization, in synapses, of protein I, an endogenous substrate for protein kinases in mammalian brain.蛋白质I（哺乳动物脑中蛋白激酶的内源性底物）在突触中的免疫细胞化学定位。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5982-6. doi: 10.1073/pnas.76.11.5982.
7
Widespread distribution of protein I in the central and peripheral nervous systems.蛋白质I在中枢和外周神经系统中广泛分布。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5977-81. doi: 10.1073/pnas.76.11.5977.
8
Depolarization-induced phosphorylation of specific proteins, mediated by calcium ion influx, in rat brain synaptosomes.由钙离子内流介导的大鼠脑突触体中特定蛋白质的去极化诱导磷酸化。
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Multiple phosphorylation sites in protein I and their differential regulation by cyclic AMP and calcium.蛋白质I中的多个磷酸化位点及其受环磷酸腺苷和钙的差异调节。
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5402-6. doi: 10.1073/pnas.76.10.5402.
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Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase.两种被环磷酸腺苷依赖性蛋白激酶磷酸化的蛋白质在大脑皮层中的亚细胞分布。
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