Montarras D, Poupel O, Fiszman M, Marcovich H
C R Seances Acad Sci III. 1983;297(8):423-6.
Quail embryo skin fibroblasts infected with wild type Rous sarcoma virus SR-RSV grow, at 36 or 41 degrees C, twice as fast (30 hours. mean division time) as the uninfected controls or the cells infected with RAV-1 virus. At 36 degrees C the SR-RSV infected cells stop growing after the same delay as the controls, thus having undergone twice as many doublings, (38 to 40 instead 18 to 20). However, at 41 degrees C, SR-RSV infected cells stop 5 to 6 divisions earlier. This cannot be reversed by shifting the cultures to 36 degrees C. Rising to 41 degrees C those cultures which grew previously at 36 degrees C reduce the number of residual divisions. No such effect is observed with NYts68 or PA101 viruses which display a reduced level of pp60v-src activity at 41 degrees C.
感染野生型劳斯肉瘤病毒SR-RSV的鹌鹑胚胎皮肤成纤维细胞，在36或41摄氏度下生长速度是未感染的对照细胞或感染RAV-1病毒的细胞的两倍（平均分裂时间为30小时）。在36摄氏度时，感染SR-RSV的细胞与对照细胞经过相同延迟后停止生长，因此经历的倍增次数是对照细胞的两倍（38至40次，而不是18至20次）。然而，在41摄氏度时，感染SR-RSV的细胞提前5至6次分裂停止生长。将培养物转移到36摄氏度无法逆转这种情况。之前在36摄氏度下生长的培养物升温至41摄氏度会减少剩余分裂次数。在41摄氏度时显示pp60v-src活性水平降低的NYts68或PA101病毒未观察到这种效应
