染色体与人类癌症和白血病的病因。LII. B细胞慢性淋巴细胞白血病患者经治疗后的染色体研究结果

Chromosomes and causation of human cancer and leukemia. LII. Chromosome findings in treated patients with B-cell chronic lymphocytic leukemia.

作者信息

Sadamori N, Han T, Minowada J, Sandberg A A

出版信息

Cancer Genet Cytogenet. 1984 Feb;11(2):161-8. doi: 10.1016/0165-4608(84)90110-9.

Abstract

The chromosomes of stimulated lymphocytes in 40 treated cases of B-cell chronic lymphocytic leukemia (B-CLL) were examined. The polyclonal B-cell activators (PBA) used were: pokeweed mitogen (PWM), Epstein-Barr virus (EBV), and lipopolysaccharide W from Escherichia coli 055:B5 (LPS). Thirty-three (83%) of the 40 cases contained an adequate number of metaphases that were suitable for banding. Of 15 cases with abnormal clones, 7 cases had trisomy #12. Occasionally, trisomy #1, 6q-, i(7q), 14q+, trisomy #16, and trisomy #18 were seen. In 5 cases, marker chromosomes of unknown origin existed. The findings indicate that trisomy #12 may be a unique and nonrandom karyotypic change in B-CLL.

摘要

对40例经治疗的B细胞慢性淋巴细胞白血病（B-CLL）患者受刺激淋巴细胞的染色体进行了检查。使用的多克隆B细胞激活剂（PBA）有：商陆有丝分裂原（PWM）、爱泼斯坦-巴尔病毒（EBV）以及来自大肠杆菌055:B5的脂多糖W（LPS）。40例患者中有33例（83%）含有足够数量适合显带的中期分裂相。在15例有异常克隆的病例中，7例存在12号染色体三体。偶尔可见1号染色体三体、6号染色体长臂缺失、7号染色体长臂等臂染色体、14号染色体长臂增加、16号染色体三体以及18号染色体三体。5例存在来源不明的标记染色体。这些发现表明，12号染色体三体可能是B-CLL中一种独特且非随机的核型改变。